IHMS_SOP 010 V2
Standard Operating Procedure for DNA short reads Quality Control Genoscope Evry

Publications

• BMC Microbiology - 2012

  Storage conditions of intestinal microbiota matter in metagenomic analysis

  Though this preliminary work explored a very limited number of parameters, the results suggest that storage conditions of fecal samples affect the integrity of DNA and RNA and the composition of their microbial community. For optimal preservation, stool samples should be kept at room temperature and brought at the laboratory within 24 h after collection or be stored immediately at −20°C in a home freezer and transported afterwards in a freezer pack to ensure that they do not defrost at any time. Mixing the samples with RNAse inhibitors outside the laboratory is not recommended since proper homogenization of the stool is difficult to monitor

  DOI:10.1186/1471-2180-12-1583

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• BMC Microbiology - 2014

  Processing faecal samples: a step forward for standards in microbial community analysis

  The degree of hydration and homogenisation of faecal samples do not significantly alter their microbial community composition. However, the use of bead-beating is critical for the proper detection of Gram-positive bacteria such as Blautia and Bifidobacterium

  DOI:10.1186/1471-2180-14-112

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